Transgenic Animal Technology

by Pinkert

• ISBN13: 9780125571661
• ISBN10: 0125571666
• Edition: 2nd
• Format: Hardcover
• Copyright: 2002-11-18
• Publisher: Elsevier Science

Summary

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer from molecular methods to whole animal considerations for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Table of Contents

Contributors	xi
Preface	xv
PART 1 Overview
Introduction to Transgenic Animal Technology
Carl A. Pinkert
Introduction	3	(1)
Historical Background	4	(3)
Applications and Overview of Text	7	(8)
References	11	(4)
PART 2 Transgenic Animal Production Focusing on the Mouse Model
DNA Microinjection and Transgenic Animal Production
H. G. Polities
Carl A. Pinkert
Introduction	15	(1)
General Methods	16	(46)
Summary	62	(10)
Acknowledgments	62	(1)
References	63	(2)
Appendix	65	(7)
Factors Affecting Transgenic Animal Production
Paul A. Overbeek
Introduction	72	(1)
Getting Started	72	(20)
Troubleshooting	92	(6)
Transgenic Phenomenology	98	(10)
Summary	108	(5)
Acknowledgments	109	(1)
References	109	(4)
Gene Targeting in Embryonic Stem Cells: I History and Methodology
Thomas Doetschman
Stem Cells of the Embryo	113	(8)
Gene Targeting in Embryonic Stem Cells	121	(6)
Methods	127	(17)
Acknowledgments	136	(1)
References	136	(8)
Gene Targeting in Embryonic Stem Cells: II Conditional Technologies
Edmund B. Rucker III
James G. Thomson
Jorge A. Piedrahita
Introduction	144	(1)
Conditional Modeling: A Brief History	145	(1)
Cre-loxP System Review, Development and Testing	146	(2)
Cre-loxP and Transgenic Models	148	(12)
Methods	160	(4)
Summary	164	(9)
Acknowledgments	166	(1)
References	166	(7)
Retrovirus-Mediated Gene Transfer
Teoan Kim
Introduction and Discussion	173	(12)
Methods	185	(10)
Acknowledgment	190	(1)
References	190	(5)
Nuclear Transfer Technologies
Yukio Tsunoda
Yoko Kato
Introduction and Discussion	195	(5)
Materials and Equipment	200	(4)
Preparation of Microtools	204	(2)
Nuclear Transfer of Preimplantation Embryos	206	(15)
Nuclear Transfer of Embryonic Stem Cells and Somatic Cells	221	(14)
Acknowledgment	229	(1)
References	229	(6)
PART 3 Production of Transgenic Laboratory and Domestic Animal Species
Production of Transgenic Rats
Philip Iannaccone
Vasility Galat
Introduction	235	(1)
Importance of Rats	236	(1)
Contributions from Transgenic Rats	237	(2)
Production	239	(8)
Conclusions	247	(4)
References	247	(4)
Production of Transgenic Rabbits
James M. Robl
Amy S. Burnside
Applications of Transgenic Rabbits	251	(2)
Manipulation of Rabbit Embryos for Production of Transgenics	253	(1)
Materials and Methods	253	(8)
References	259	(2)
Production of Transgenic Fish
Richard N. Winn
Introduction	261	(1)
Discussion	262	(1)
Methods of Gene Transfer	263	(9)
Establishing and Maintaining Transgenic Lineages	272	(3)
Care and Containment of Transgenic Fish	275	(1)
Future of the Transgenic Fish	276	(3)
Acknowledgments	276	(1)
References	276	(3)
Production of Transgenic Poultry
J. N. Petitte
P. E. Mozdziak
Introduction and Discussion	279	(9)
Methods	288	(15)
Summary	303	(5)
References	303	(5)
Production of Transgenic Swine by DNA Microinjection
M. J. Martin
Carl A. Pinkert
Introduction and Discussion	308	(5)
Methods	313	(10)
Alternative Methods for the Production of Transgenic Swine	323	(5)
Summary	328	(9)
References	328	(9)
Production of Transgenic Ruminants by DNA Microinjection
H. Niemann
H. H. Dopke
K. G. Hadeler
Introduction	337	(1)
Production of Zygotes Suitable for Microinjection	338	(6)
Microinjection of Ruminant Zygotes	344	(5)
Transfer of Microinjected Embryos	349	(5)
Areas of Application and Perspectives	354	(5)
Acknowledgments	355	(1)
References	355	(4)
Production of Transgenic Nonhuman Primates
A. W. S. Chan
K. Y. Chong
G. Schatten
Introduction	359	(9)
Methods	368	(13)
Summary	381	(14)
Acknowledgments	381	(1)
References	382	(13)
Nuclear Transfer Technology in Cattle, Sheep, and Swine
Lesley Paterson
William Ritchie
Ian Wilmut
Introduction	395	(1)
General Methods	396	(18)
Summary	414	(6)
Acknowledgments	414	(1)
References	414	(6)
PART 4 Molecular Biology, Analyses, and Enabling Technologies
Vector Design for Transgene Expression
L. M. Houdebine
J. Attal
J. L. Vilotte
Introduction	420	(1)
Transcribed Region	421	(11)
Promoter Region	432	(1)
Boundary Regions	433	(13)
Conclusions and Perspective	446	(13)
Acknowledgment	448	(1)
References	448	(11)
Analysis of Transgene Integration
Brad T. Tinkle
Gilbert Jay
Introduction	459	(1)
Detection of the Transgene	459	(2)
Parameters for Evaluating a Transgene	461	(1)
Analysis of the Transgene	462	(5)
Establishing a Transgenic Line	467	(2)
Derivation of Homozygous Mice	469	(1)
Summary	470	(5)
References	471	(4)
PCR Optimization for Detection of Transgene Integration
M. H. Irwin
W. K. Pogozelski
Carl A. Pinkert
Introduction	475	(1)
Discussion	476	(7)
Summary	483	(3)
References	483	(3)
Analysis of Transgene Expression
Lien Ngo
Gilbert Jay
Introduction	486	(2)
Analysis of Steady-State Levels of Transgenic RNA Transcripts	488	(11)
Analysis of Steady-State Levels of Transgenic Protein Products	499	(11)
Conclusions	510	(4)
References	510	(4)
Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals
Robert A. Godke
Marina Sansinena
Curtis R. Youngs
Introduction	514	(1)
Assisted Reproductive Technologies	515	(34)
Development of New Assisted Reproductive Technologies	549	(3)
Summary	552	(17)
References	554	(15)
Databases, Internet Resources, and Genetic Nomenclature
Anna V. Anagnostopoulos
Lary E. Mobraaten
Muriel T. Davisson
Introduction	569	(1)
Transgenic and Targeted Mutation Databases and Electronic Resources	570	(14)
Standard Nomenclature for Transgenic and Targeted Mutation Animals	584	(17)
References	589	(3)
Appendix I: Additional Mouse Databases and Internet Resources	592	(4)
Appendix II: Rat Database and Internet Resources	596	(1)
Appendix III: Swine Database and Internet Resources	597	(1)
Appendix IV: Sheep Database and Internet Resources	598	(1)
Appendix V: Cattle Database and Internet Resources	598	(1)
Appendix VI: Poultry Database and Internet Resources	599	(1)
Appendix VII: Fish Database and Internet Resources	599	(2)
Index	601