9780125571661

Transgenic Animal Technology

by
  • ISBN13:

    9780125571661

  • ISBN10:

    0125571666

  • Edition: 2nd
  • Format: Hardcover
  • Copyright: 2002-11-18
  • Publisher: Elsevier Science

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

Purchase Benefits

  • Free Shipping On Orders Over $35!
    Your order must be $35 or more to qualify for free economy shipping. Bulk sales, PO's, Marketplace items, eBooks and apparel do not qualify for this offer.
  • Get Rewarded for Ordering Your Textbooks! Enroll Now
List Price: $127.00 Save up to $31.75
  • Buy Used
    $95.25
    Add to Cart Free Shipping

    USUALLY SHIPS IN 2-4 BUSINESS DAYS

Supplemental Materials

What is included with this book?

Summary

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer from molecular methods to whole animal considerations for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Table of Contents

Contributors xi
Preface xv
PART 1 Overview
Introduction to Transgenic Animal Technology
Carl A. Pinkert
Introduction
3(1)
Historical Background
4(3)
Applications and Overview of Text
7(8)
References
11(4)
PART 2 Transgenic Animal Production Focusing on the Mouse Model
DNA Microinjection and Transgenic Animal Production
H. G. Polities
Carl A. Pinkert
Introduction
15(1)
General Methods
16(46)
Summary
62(10)
Acknowledgments
62(1)
References
63(2)
Appendix
65(7)
Factors Affecting Transgenic Animal Production
Paul A. Overbeek
Introduction
72(1)
Getting Started
72(20)
Troubleshooting
92(6)
Transgenic Phenomenology
98(10)
Summary
108(5)
Acknowledgments
109(1)
References
109(4)
Gene Targeting in Embryonic Stem Cells: I History and Methodology
Thomas Doetschman
Stem Cells of the Embryo
113(8)
Gene Targeting in Embryonic Stem Cells
121(6)
Methods
127(17)
Acknowledgments
136(1)
References
136(8)
Gene Targeting in Embryonic Stem Cells: II Conditional Technologies
Edmund B. Rucker III
James G. Thomson
Jorge A. Piedrahita
Introduction
144(1)
Conditional Modeling: A Brief History
145(1)
Cre-loxP System Review, Development and Testing
146(2)
Cre-loxP and Transgenic Models
148(12)
Methods
160(4)
Summary
164(9)
Acknowledgments
166(1)
References
166(7)
Retrovirus-Mediated Gene Transfer
Teoan Kim
Introduction and Discussion
173(12)
Methods
185(10)
Acknowledgment
190(1)
References
190(5)
Nuclear Transfer Technologies
Yukio Tsunoda
Yoko Kato
Introduction and Discussion
195(5)
Materials and Equipment
200(4)
Preparation of Microtools
204(2)
Nuclear Transfer of Preimplantation Embryos
206(15)
Nuclear Transfer of Embryonic Stem Cells and Somatic Cells
221(14)
Acknowledgment
229(1)
References
229(6)
PART 3 Production of Transgenic Laboratory and Domestic Animal Species
Production of Transgenic Rats
Philip Iannaccone
Vasility Galat
Introduction
235(1)
Importance of Rats
236(1)
Contributions from Transgenic Rats
237(2)
Production
239(8)
Conclusions
247(4)
References
247(4)
Production of Transgenic Rabbits
James M. Robl
Amy S. Burnside
Applications of Transgenic Rabbits
251(2)
Manipulation of Rabbit Embryos for Production of Transgenics
253(1)
Materials and Methods
253(8)
References
259(2)
Production of Transgenic Fish
Richard N. Winn
Introduction
261(1)
Discussion
262(1)
Methods of Gene Transfer
263(9)
Establishing and Maintaining Transgenic Lineages
272(3)
Care and Containment of Transgenic Fish
275(1)
Future of the Transgenic Fish
276(3)
Acknowledgments
276(1)
References
276(3)
Production of Transgenic Poultry
J. N. Petitte
P. E. Mozdziak
Introduction and Discussion
279(9)
Methods
288(15)
Summary
303(5)
References
303(5)
Production of Transgenic Swine by DNA Microinjection
M. J. Martin
Carl A. Pinkert
Introduction and Discussion
308(5)
Methods
313(10)
Alternative Methods for the Production of Transgenic Swine
323(5)
Summary
328(9)
References
328(9)
Production of Transgenic Ruminants by DNA Microinjection
H. Niemann
H. H. Dopke
K. G. Hadeler
Introduction
337(1)
Production of Zygotes Suitable for Microinjection
338(6)
Microinjection of Ruminant Zygotes
344(5)
Transfer of Microinjected Embryos
349(5)
Areas of Application and Perspectives
354(5)
Acknowledgments
355(1)
References
355(4)
Production of Transgenic Nonhuman Primates
A. W. S. Chan
K. Y. Chong
G. Schatten
Introduction
359(9)
Methods
368(13)
Summary
381(14)
Acknowledgments
381(1)
References
382(13)
Nuclear Transfer Technology in Cattle, Sheep, and Swine
Lesley Paterson
William Ritchie
Ian Wilmut
Introduction
395(1)
General Methods
396(18)
Summary
414(6)
Acknowledgments
414(1)
References
414(6)
PART 4 Molecular Biology, Analyses, and Enabling Technologies
Vector Design for Transgene Expression
L. M. Houdebine
J. Attal
J. L. Vilotte
Introduction
420(1)
Transcribed Region
421(11)
Promoter Region
432(1)
Boundary Regions
433(13)
Conclusions and Perspective
446(13)
Acknowledgment
448(1)
References
448(11)
Analysis of Transgene Integration
Brad T. Tinkle
Gilbert Jay
Introduction
459(1)
Detection of the Transgene
459(2)
Parameters for Evaluating a Transgene
461(1)
Analysis of the Transgene
462(5)
Establishing a Transgenic Line
467(2)
Derivation of Homozygous Mice
469(1)
Summary
470(5)
References
471(4)
PCR Optimization for Detection of Transgene Integration
M. H. Irwin
W. K. Pogozelski
Carl A. Pinkert
Introduction
475(1)
Discussion
476(7)
Summary
483(3)
References
483(3)
Analysis of Transgene Expression
Lien Ngo
Gilbert Jay
Introduction
486(2)
Analysis of Steady-State Levels of Transgenic RNA Transcripts
488(11)
Analysis of Steady-State Levels of Transgenic Protein Products
499(11)
Conclusions
510(4)
References
510(4)
Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals
Robert A. Godke
Marina Sansinena
Curtis R. Youngs
Introduction
514(1)
Assisted Reproductive Technologies
515(34)
Development of New Assisted Reproductive Technologies
549(3)
Summary
552(17)
References
554(15)
Databases, Internet Resources, and Genetic Nomenclature
Anna V. Anagnostopoulos
Lary E. Mobraaten
Muriel T. Davisson
Introduction
569(1)
Transgenic and Targeted Mutation Databases and Electronic Resources
570(14)
Standard Nomenclature for Transgenic and Targeted Mutation Animals
584(17)
References
589(3)
Appendix I: Additional Mouse Databases and Internet Resources
592(4)
Appendix II: Rat Database and Internet Resources
596(1)
Appendix III: Swine Database and Internet Resources
597(1)
Appendix IV: Sheep Database and Internet Resources
598(1)
Appendix V: Cattle Database and Internet Resources
598(1)
Appendix VI: Poultry Database and Internet Resources
599(1)
Appendix VII: Fish Database and Internet Resources
599(2)
Index 601

Rewards Program

Write a Review