Transgenic Fish

by Hew, Choy L.; Fletcher, Garth L.

• ISBN13: 9789810209971
• ISBN10: 9810209975
• Format: Hardcover
• Copyright: 1992-09-01
• Publisher: World Scientific Pub Co Inc

Summary

Studies on transgenic fish, in contrast to mammals, are still in their infancy. However, it is evident that such fish will not only be of considerable economic benefit to aquaculture but will enable scientists to make quantum leaps in their understanding of physiological and biochemical mechanisms unique to fish, and of the developmental biology of vertebrates in general.
The potential of transgenic fish for research and industrial development is beginning to be widely recognized. This timely volume encompasses the full spectrum of current research on transgenic fish. It will be valuable to many scientists who intend to explore the merits of the technology for the first time.

Table of Contents

Preface
In memory of Kevin Scott Guise
Contributors
Oogenesis, Models and Gene Transfer Techniques
Egg Development: From Gametes to Embryonic Development and Hatching	p. 1
Gene Transfer and Expression in Medaka Embryos	p. 27
Gene Transfer in Salmonids by Injection Through the Micopyle	p. 44
The Application of PCR to Aquaculture	p. 61
Medaka and Zebrafish: Ideal as Transient and Stable Transgenic Systems	p. 72
Inheritance and Expression Studies
Generation of Fast Growing Transgenic Fish: Methods and Mechanisms	p. 92
Expression of Heterologous Genes in Transgenic Fish	p. 120
Transfer of Genes Encoding Neomycin Resistance, Chloramphenicol Acetyl Transferase and Growth Hormone into Goldfish and Northern Pike	p. 142
Integration, Expression and Inheritance of Foreign Fish Growth Hormone Gene in Transgenic Fish	p. 164
Growth Hormone Gene Transfer in Atlantic Salmon: Use of Fish Antifreeze/Growth Hormone Chimeric Gene Construct	p. 176
Genetic Engineering of Freeze-Resistant Atlantic Salmon	p. 190
Ecological and Containment Considerations
Ecological and Regulatory Uncertainties Associated with Transgenic Fish	p. 209
Containment of Genetically Altered Fish with Emphasis on Salmonids	p. 229
Subject Index	p. 267
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