Triple Helix Forming Oligonucleotides

by Malvy, Claude; Harel-Bellan, Annick; Pritchard, Linda L.

• ISBN13: 9780792384182
• ISBN10: 0792384180
• Format: Hardcover
• Copyright: 1999-03-01
• Publisher: Kluwer Academic Pub

Summary

Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.

Table of Contents

Foreword

Background and Structural Aspects

The Antigene Strategy: Progress and Perspectives in Selective Gene Silencing

Triple-Helix Structure

The Triple-Helix-Forming Oligonucleotide

Thermodynamic State Diagrams of Oligonucleotide Triple Helices

Imaging of Triplexes by Electron and Scanning Force Microscopy

Chemical Modifications of Triple Helix Forming Oligonucleotides

Triple Helix Formation with Modified Oligonucleotides

Oligonucleotides as Artificial DNases

The Biology of Triple Helices

Potential Mechanisms of Action

Oligonucleotide Uptake and Delivery in Tissue Culture Cells

Triple-Stranded Complexes as Antigene Molecules: Transcription Inhibition Ex Vivo

Gene-Targeting Triple Helix Forming Purine Oligonucleotides

DNA Triple Helix as a Tool to Regulate Cytokine Gene Expression

Genome Modification by Triplex-Forming Oligonucleotides

Psoralen-Coupled Oligonucleotides: In Vivo Binding and Repair

Structure and Biology of H DNA

Triplex-Binding Proteins

RNA and Triple Helices

New Developments and Applications

Triplexes Involving PNA

Triple Helix Stabilizing Agents

New Targets for Triple Helix Forming Oligonucleotides

Triplexes and Biotechnology

Index

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