Viral Genome Methods

by Adolph; Kenneth W.

• ISBN13: 9780849344121
• ISBN10: 0849344123
• Edition: 1st
• Format: Paperback
• Copyright: 1996-08-19
• Publisher: CRC Press

Summary

Molecular biology and genetics techniques now dominate viral research in attempts to cure diseases such as AIDS. Viral Genome Methods is a practical guide to the newest molecular techniques, providing step-by-step protocols to be used in the laboratory. Recognized authorities and pioneers in viral research pass on their expertise to you.

Table of Contents

Section I. Retroviruses/HIV/RNA Viruses	3	(200)
Chapter 1. Retrovirus-Mediated Gene Transfer	3	(22)
Bruce A. Bunnell
Richard A. Morgan
Chapter 2. Methods for Studying Genetic Variation of the Human Immunodeficiency Virus (HIV)	25	(18)
Gerald Schochetman
Shambavi Subbarao
Marcia L. Kalish
Chapter 3. Methods for HIV/SIV Gene Analysis	43	(12)
Akio Adachi
Meiko Kawamura
Kenzo Tokunaga
Hiroyuki Sakai
Chapter 4. Analysis of Human Immunodeficiency Virus Reverse Transcriptase Function	55	(20)
Vinayaka R. Prasad
William C. Drosopoulos
Yvonne Kew
Chapter 5. Tat Activation Studies: Use of a Reporter Cell Line	75	(14)
Estuardo Aguilar-Cordova
Javier Chinen
John W. Belmont
Chapter 6. Characterization of HIV Nef Cell-Specific Effects	89	(22)
Jon W. Marsh
Swapan K. De
Chapter 7. Detection, Quantitation, and Characterization of Human Retroviruses	111	(56)
Suraiya Rasheed
Chapter 8. Quantitative Methods to Monitor Viral Load in Simian Immunodeficiency Virus Infections	167	(18)
Silvija I. Staprans
Brian C. Corliss
Jessica L. Guthrie
Mark B. Feinberg
Chapter 9. Using Molecular Genetics to Probe the Immune Response to Vesicular Stomatitis Virus	185	(18)
Carol S. Reiss
Shelli A. Oien
Shirley M. Bartido
Section II. DNA Viruses	203	(80)
Chapter 10. Transient Genetic Approaches to Studying Multifactor Processes in Complex DNA Viruses	203	(16)
Gregory S. Pari
Andrea C. Iskenderian
David G. Anders
Chapter 11. Epstein-Barr Virus: Gene Expression and Regulation	219	(22)
Nirupama Deshmane Sista
Joseph S. Pagano
Chapter 12. Functional Analysis of the Herpes Simplex Virus DNA Polymerase	241	(12)
Paul Digard
William Bebrin
Connie Chow
Donald M. Coen
Chapter 13. Molecular Genetics of Adeno-Associated Virus	253	(16)
Barrie J. Carter
Chapter 14. Reconstitution of Adenovirus DNA Replication with Purified Replication Proteins	269	(14)
Job Dekker
Frank E. J. Coenjaerts
Peter C. van der Vliet
Section III. Index	283