In Vitro Methods in Toxicology

by Edited by C. K. Atterwill , C. E. Steele

• ISBN13: 9780521104753
• ISBN10: 0521104750
• Edition: 1st
• Format: Paperback
• Copyright: 2009-03-19
• Publisher: Cambridge University Press

Summary

First published in 1987, this book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies. This type of research has important ethical, financial and scientific implications. In particular, less animals and compound are required in in vitro research and more detailed information at the cellular and subcellular levels can be obtained. The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists. It could also form a framework for discussion of potential improvements and modification of toxicity testing.

Table of Contents

Introduction

Target Organ Toxicity

In vitro methods in renal toxicology

Ototoxicity

Cultured cell models for studying problems in cardiac toxicology

Use of the isolated rat heart for the detection of cardiotoxic agents

Brain reaggregate cultures in neurotoxicological investigations

Application of thyroid cell culture to the study of thyrotoxicity

In vitro methods to investigate toxic lung disease

Metabolism and toxicity of drugs in mammalian hepatocyte culture

In vitro evaluation of haemic systems in toxicology

General and Topical Toxicity

The chorioallantoic membrance in irritancy testing

In vitro dermal irritancy tests

Genetic toxicology

In vitro immunotoxicology

Reproductive Toxicity

An in vitro test for teratogens using cultures of rat embryo cells

Postimplantion embryo culture and its application to problems in teratology

Sub-mammalian and sub-vertebrate models in teratogenicity screening

The use of in vitro techniques to investigate the action of testicular toxicants

An in vitro assessment of ovarian function: a potential tool for investigating mechanisms of toxicity in the ovary

Conclusion

Conclusion

Index

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