Wood Formation in Trees : Cell and Molecular Biology Techniques

by Chaffey, Nigel J.

• ISBN13: 9780203166444
• ISBN10: 0203166442
• eBook ISBN(s): 9781134485062
• Copyright: 2004-01-14
• Publisher: Taylor & Francis
• More Book Details

Summary

Trees, a major component of the biosphere, can provide solutions to modern challenges of global warming and dwindling fossil fuel reserves. Unfortunately, too little is known about the biology of these plants, due to a lack of appropriate techniques for studying how they are formed. Wood Formation in Trees presents a variety of detailed protocols for the study of the cell and molecular biology of wood formation: anatomical, biochemical and molecular-genetic. Internationally recognized experts, most of whom are the researchers who developed the techniques, speak with authority in this volume, and also provide first-hand tips and trade secrets to help the uninitiated master the techniques.

Table of Contents

Introduction

An Introduction to the Problems of Working with Trees

Wood Microscopical Techniques

Conventional (Chemical-fixation) Transmission Electron Microscopy and Cytochemistry of Angiosperm Trees

Chemical and Cryo-fixation for Transmission Electron Microscopy of Gymnosperm Cambial Cells

Deep-etching Electron Microscopy and 3-Dimensional Cell Wall Architecture

Secondary Ion Mass Spectometry Microscopy: Application to the Analysis of Woody Samples

Immunolocalization of the Cytoskeleton in the Secondary Vascular System of Angiosperm Trees and its Visualisation Using Epifluorescence Microscopy

Immunolocalization and Visualization of the Cytoskeleton in Gymnosperms using Confocal Laser Scanning Microscopy

Cell Walls of Woody Plants: Autoradiography and Ultraviolet Microscopy

Cell Walls of Woody Tissues: Cytochemical, Biochemical and Molecular Analysis of Pectins and Pectin Methylesterases

Immunolocalization of Enzymes of Lignification

Sampling of Cambial Region Tissues for High Resolution Analysis

Biochemistry and Quantitative Histochemistry of Wood

Protein Analysis in Perennial Tissues

The Use of GUS Histochemistry to Visualize Lignification Gene Expression In Situ during Wood Formation

In Situ Hybridization

Random Amplification of Polymorphic DNA and Reverse transcription Chain Reaction of RNA in Studies of Sapwood and Heartwood

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