Wood Formation in Trees: Cell and Molecular Biology Techniques

by Chaffey; Nigel J

• ISBN13: 9780415272155
• ISBN10: 0415272157
• Edition: 1st
• Format: Hardcover
• Copyright: 2002-01-10
• Publisher: CRC Press

Summary

Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of appropriate techniques. In recognition of this, Wood Formation in Trees presents a variety of detailed techniques and protocols for the study of the cell and molecular biology of wood formation in trees. Internationally recognized experts, most of whom are the researchers who developed the techniques, speak with authority in this volume, and also provide first-hand tips and trade secrets to help the uninitiated master the techniques. The techniques reflect a hierarchical approach to the study of the developmental biology of wood formation: anatomical, biochemical and molecular-genetic.Trees are a tremendous but vastly under-appreciated natural resource. In an age where the natural product is so often modified to suit modern tastes and industrial processes, it is essential to understand how the natural product is made. The techniques in this book provide that essential information about the process of wood formation in trees.

Table of Contents

Foreword	vii
Anne-Marie Catesson
Preface	ix
Contributors	xi
List of Abbreviations	xv
Introduction	1	(8)
Nigel Chaffey
An Introduction to the Problems of Working with Trees	9	(8)
Nigel Chaffey
Wood Microscopical Techniques	17	(24)
Nigel Chaffey
Conventional (Chemical-fixation) Transmission Electron Microscopy and Cytochemistry of Angiosperm Trees	41	(24)
Nigel Chaffey
Chemical and Cryo-fixation for Transmission Electron Microscopy of Gymnosperm Cambial Cells	65	(18)
Kim H. Rensing
Deep-etching Electron Microscopy and 3-dimensional Cell Wall Architecture	83	(16)
Takao Itoh
Secondary Ion Mass Spectrometry Microscopy: Application to the Analysis of Woody Samples	99	(14)
Marie-Laure Follet-Gueye
Immunolocalisation of the Cytoskeleton in the Secondary Vascular System of Angiosperm Trees and its Visualization Using Epifluorescene Microscopy	113	(30)
Nigel Chaffey
Immunolocalisation and Visualisation of the Cytoskeleton in Gymnosperms using Confocal Laser Scanning Microscopy	143	(16)
Ryo Funada
Cell Walls of Woody Plants: Autoradiography and Ultraviolet Microscopy	159	(20)
Keiji Takabe
Cell Walls of Woody Tissues: Cytochemical, Biochemical and Molecular Analysis of Pectins and Pectin Methylesterases	179	(22)
Fabienne Micheli
Fabienne E. Ermel
Marianne Bordenave
Luc Richard
Renee Goldberg
Immunolocalisation of Enzymes of Lignification	201	(14)
Jozef Samaj
Alain Michel Boudet
Sampling of Cambial Region Tissues for High Resolution Analysis	215	(14)
Claes Uggla
Bjorn Sundberg
Biochemistry and Quantitative Histochemistry of Wood	229	(16)
Elisabeth Magel
Protein Analysis in Perennial Tissues	245	(26)
Tannis Beardmore
Suzanne Wetzel
Carrie-Ann Whittle
The Use of GUS Histochemistry to Visualise Lignification Gene Expression In Situ during Wood Formation	271	(26)
Simon Hawkins
Gilles Pilate
Eric Duverger
Alain Michel Boudet
Jacqueline Grima-Pettenati
High Resolution In Situ Hybridisation in Woody Tissues	297	(22)
Sharon Regan
Bjorn Sundberg
Random Amplification of Polymorphic DNA and Reverse Transcription Polymerase Chain Reaction of RNA in Studies of Sapwood and Heartwood	319	(20)
Elisabeth Magel
Siegfried Hauch
Luigi De Filippis
List of Suppliers	339	(10)
List of Taxa	349	(2)
Index	351