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9780122121722

The Zebrafish: Genetics and Genomics

by ;
  • ISBN13:

    9780122121722

  • ISBN10:

    0122121724

  • Format: Paperback
  • Copyright: 1999-01-01
  • Publisher: Academic Pr
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List Price: $185.00

Summary

This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Is relevant to clinicians interested in vertebrate models of human congenital diseases

Table of Contents

Contributors
Preface
A Gynogenesis-Based Screen for Maternal-Effect Genes in the Zebrafish
Developmental Mutant Screens in the Zebrafish
Haploid Screens and Gamma-Ray Mutagenesis
Early Pressure Screens
Retrovirus-Mediated Insertional Mutagenesis in Zebrafish
Genetic Applications of Transposons and Other Repetitive Elements in Zebrafish
Transgenesis
The Zebrafish Genome
Using Random Amplified Polymorphic DNAs in Zebrafish Genomic Analysis
Simple Sequence-Length Polymorphism Analysis
Gene Mapping in Zebrafish Using Single-Strand Conformation Polymorphism Analysis
Mapping Zebrafish Mutations by AFLP
Zebrafish Expressed Sequence Tags and Their Applications
Positional Cloning of Mutated Zebrafish Genes
Construction and Characterization of Zebrafish Whole Genome Radiation Hybrids
Zebrafish/Mouse Somatic Cell Hybrids for the Characterization of the Zebrafish Genome
Banded Chromosomes and the Zebrafish Karyotype
Zebrafish Informatics and the ZFIN Database
Genetic Backgrounds and Some Standard Stocks and Strains Used in Zebrafish Developmental Biology and Genetics
Centromeric Markers in the Zebrafish
Collection, Storage, and Use of Zebrafish Sperm
Zebrafish Web Site Listings
Index
Table of Contents provided by Publisher. All Rights Reserved.

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