Microbiological Applications : A Laboratory Manual in General Microbiology, Complete Version

by Benson, Harold J.; Pasadena City College

• ISBN13: 9780072318883
• ISBN10: 0072318880
• Edition: 8th
• Format: Paperback
• Copyright: 2001-07-24
• Publisher: MCG

Summary

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Benson's Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.

Table of Contents

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Protozoa, Algae, and Cyanobacteria

7 Microscopic Invertebrates

8 Aseptic Technique

9 The Bacteria

10 The Fungi: Yeasts and Molds

Part 3 Microscope Slide Techniques

11 Negative Staining

12 Smear Preparation

13 Simple Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Ziehl-Neelsen Method

18 Acid-Fast Staining: Fluorescence Method

19 Motility Determination

Part 4 Culture Methods

20 Culture Media Preparation

21 Pure Culture Techniques

22 Cultivation of Anaerobes

23 Bacterial Population Counts

24 Slide Culture: Autotrophs

25 Slime Mold Culture

26 Slide Culture: Molds

27 Isolation of Anaerobic Phototrophic Bacteria: Using the Winogradsky Column

Part 5 Bacterial Viruses

28 Isolation of Phage from Sewage

29 Isolation of Phage from Flies

30 Burst Size Determination: A One-Step Growth Curve

Part 6 Microbial Interrelationships

31 Bacterial Commensalism

32 Bacterial Synergism

33 Microbial Anatagonism

Part 7 Environmental Influences and Control of Microbial Growth

34 Temperature: Effects on Growth

35 Temperature: Lethal Effects

36 pH and Microbial Growth

37 Osmotic Pressure and Bacterial Growth

38 Oligodynamic Action

39 Ultraviolet Light: Lethal Effects

40 Evaluation of Disinfectants: The Use-Dilution Method

41 Evaluation of Alcohol: Its Effectiveness as a Skin Degerming Agent

42 Evaluation of Antiseptics: The Filter Paper Disk Agent

43 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

44 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

45 Preparation and Care of Stock Cultures

46 Morphological Study of Unknown

47 Cultural Characteristics

48 Physiological Characteristics: Oxidation and Fermentation Tests

49 Physiological Characteristics: Hydrolytic Reactions

50 Physiological Characteristics: Miscellaneous Tests

51 Use of Bergey’s Manual and Identibacter Interactus

Part 9 Miniaturized Multitest Systems

52 Enterobacteriaceae Identification: The API 20E System

53 Enterobacteriaceae Identification: The Enterotube II System

54 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

55 Staphylococcus Identification: The API Staph-Ident System

Part 10 Microbiology of Soil

56 Microbial Population Counts of Soil

57 Isolation of an Antibiotic Producer from Soil

58 The Nitrogen Cycle

59 Nitrogen-Fixing Bacteria

60 Ammonification in Soil

61 Isolation of a Denitrifier from Soil: Using Nitrate Agar

62 Isolation of a Denitrifier from Soil: Using an Enrichment Medium

Part 11 Microbiology of Water

63 Bacteriological Examination of Water: Qualitative Tests

64 The Membrane Filter Method

65 Standard Plate Count: A Quantitative Test

Part 12 Microbiology of Milk and Food Products

66 Standard Plate Count of Milk

67 Direct Microscopic Count of Organisms in Milk: The Breed Count

68 The Reductase Test

69 Bacterial Counts of Foods

70 Microbial Spoilage of Canned Foods

71 Microbial Spoilage of Refrigerated Meat

72 Microbiology of Alcohol Fermentation

73 Microbiology of Yogurt Production

Part 13 Bacterial Genetic Variations

74 Mutant Isolation of Gradient Plate Method

75 Mutant Isolation by Replica Plating

76 Bacterial Mutagenicity and Carcinogenesis: The Ames Test

Part 14 Medical Microbiology and Immunology

77 A Synthetic Epidemic

78 The Staphylococci: Isolation and Identification

79 The Streptococci: Isolation and Identification

80 Gram-Negative Intestinal Pathogens

81 Urinary Tract Pathogens

82 Slide Agglutination Test: Serological Typing

83 Slide Agglutination (Latex) Test: For S. aureus Identification

84 Tube Agglutination Test: The Heterophile Antibody Test

85 Tube Agglutination Test: The Widal Test

86 Phage Typing

87 White Blood Cell Study: The Differential WBC Count

88 Total WBC Count

89 Blood Grouping

90 The Snyder Caries Susceptibility Test

Appendix A Tables

Appendix B Indicators, Stains, Reagents

Appendix C Media

Appendix D Identificaiton Charts

Appendix E The Streptococci

Appendix F Identibacter interactus