Principles of Fluorescence Spectroscopy

by Lakowicz, J.

• ISBN13: 9780306460937
• ISBN10: 0306460939
• eBook ISBN(s): 9781475730616
• Additional ISBN(s): 9780387312781
• Edition: 2nd
• Format: Hardcover
• Copyright: 1999-07-01
• Publisher: Kluwer Academic Pub
• More Book Details
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Summary

'In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Table of Contents

Introduction to Fluorescence

Instrumentation for Fluorescence Spectroscopy

Fluorophores

Time-Domain Lifetime Measurements

Frequency-Domain Lifetime Measurements

Solvent Effects on Emission Spectra

Dynamics of Solvent and Spectral Relaxation

Quenching of Fluorescence

Advanced Topics in Fluorescence Quenching

Fluorescence Anisotropy

Time-Dependent Anisotropy Decays

Advanced Anisotropy Concepts

Energy Transfer

Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers

Energy Transfer to Multiple Acceptors in One, Two or Three-Dimensions

Protein Fluorescence

Time-Resolved Protein Fluorescence

Excited State Reactions

Fluorescence Sensing

Long Lifetime Metal-Ligand Complexes

DNA Technology

Phase Sensitive and Phase Resolved Emission Spectra

Appendixes

Corrected Emission Spectra

Fluorescent Lifetime Standards

Additional Reading

Index

Table of Contents provided by Publisher. All Rights Reserved.