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Preface | p. v |
Contributors | p. ix |
Molecular Biology | |
Retroviral Delivery of ECM Genes | p. 3 |
Tissue-Specific KO of ECM Proteins | p. 15 |
Recombinant Collagen Trimers from Insect Cells and Yeast | p. 51 |
Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag | p. 63 |
Preparation of Recombinant Fibronectin Fragments for Functional and Structural Studies | p. 73 |
Biochemical and Biophysical Analysis | |
Quantitative Determination of Collagen Cross-links | p. 103 |
ECM Macromolecules: Height-Mapping and Nano-Mechanics Using Atomic Force Microscopy | p. 123 |
Atomic Force Microscopy Measurements of Intermolecular Binding Forces | p. 143 |
Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy | p. 151 |
Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry | p. 163 |
ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy | p. 175 |
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates | p. 183 |
Solid Phase Assays for Studying ECM Protein-Protein Interactions | p. 195 |
Cell Biology Assays | |
Cell Adhesion Assays | p. 203 |
ECM Degradation Assays for Analyzing Local Cell Invasion | p. 211 |
Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration | p. 221 |
Fibrin Gel Model for Assessment of Cellular Contractility | p. 251 |
Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis) | p. 261 |
Stromagenesis During Tumorigenesis: Characterization of Tumor-Associated Fibroblasts and Stroma-Derived 3D Matrices | p. 275 |
Organ Models | |
Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes | p. 309 |
ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development | p. 319 |
Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice | p. 331 |
Tissue Engineering | |
Characterizing ECM Production by Cells Encapsulated in Hydrogels | p. 349 |
Tissue Engineering and Cell-Populated Collagen Matrices | p. 363 |
Index | p. 371 |
Table of Contents provided by Ingram. All Rights Reserved. |
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