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9780387915159

Introduction to Light Microscopy

by ;
  • ISBN13:

    9780387915159

  • ISBN10:

    038791515X

  • Format: Paperback
  • Copyright: 1998-06-01
  • Publisher: Bios Scientific Pub Ltd
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List Price: $49.95

Summary

This book provides detailed and fully illustrated advice on choosing and using the appropriate type of light microscope for a particular application. The low-power stereomicroscope is described, and the many different types of condensers, objectives and eyepieces required for the high-power compound microscope are explained in detail. The book also describes the correct care and use of the microscope in order to achieve the best possible image, and provides a checklist to aid in the diagnosis and correction of problems. Practical step-by-step guidance ensures that the reader always obtains a clear image, Introduction to Light Microscopy is therefore an essential guide for amateur and professional users of the light microscope in all areas of science.

Table of Contents

Preface xi
1. Introduction
1(24)
Why use a microscope?
1(2)
What is a microscope?
3(2)
Interactions of light with matter
5(5)
Lens aberrations
10(9)
Chromatic aberration
10(1)
Spherical aberration
11(5)
Coma
16(1)
Astigmatism
17(1)
Field curvature
18(1)
Distortion
19(1)
Scales of magnification
19(3)
Resolution and magnification
22(2)
Beyond this book
24(1)
References
24(1)
2. The hand lens
25(8)
Hand-held magnifiers
25(2)
Supported magnifiers
27(1)
Microfilm readers
28(1)
Illuminating the specimen
28(2)
Magnification
30(3)
3. Low-power stereomicroscopes
33(10)
Basic considerations
33(1)
Greenough-type stereomicroscopes
34(1)
Common-main-objective microscopes
34(2)
Macroscopes
36(1)
Supporting the instrument
36(2)
Lighting the specimen
38(2)
Image formation
40(1)
Reference
40(3)
4. Compound microscopes
43(12)
The compound microscope
43(1)
Microscopes with separate lamps
44(2)
Microscopes with built-in light sources
46(3)
Integrated microscopes
49(2)
Automated microscopes
51(2)
Specialized microscopes
53(1)
References
54(1)
5. Basic concepts: objectives and eyepieces
55(28)
More on image formation
55(3)
Conjugate planes
58(1)
Infinity-corrected systems
59(2)
Diffraction and resolution
61(8)
The objective
69(2)
Classification of objectives
71(4)
Classification according to chromatic corrections
71(3)
Dry lenses and immersion lenses
74(1)
Special types of objective
75(1)
The eyepiece
76(6)
References
82(1)
6. Illumination of the specimen
83(16)
Light sources
83(2)
The condenser
85(2)
Condenser types
87(1)
Source-focused and Kohler illumination
88(4)
Transmitted illumination
92(4)
Epi-illumination
96(2)
References
98(1)
7. Practical use of the microscope
99(20)
Getting a good image
99(2)
Changing to higher-aperture (higher-magnification) objectives
101(1)
Using a very low-power objective
101(1)
Oil-immersion objectives
102(1)
Darkground
102(1)
Reflected light or epi-illumination
103(1)
Lamp adjustment
104(2)
Setting up source-focused illumination
106(1)
Contrast enhancement
107(3)
Dissatisfaction with the microscope image
110(6)
Care of the instrument
116(2)
Reference
118(1)
Bibliography 119(2)
Index 121

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