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9780792360759

Animal Cell Technology

by ; ; ;
  • ISBN13:

    9780792360759

  • ISBN10:

    0792360753

  • Format: Hardcover
  • Copyright: 1999-12-01
  • Publisher: Kluwer Academic Pub
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Supplemental Materials

What is included with this book?

Summary

This book contains, in the form of concise papers of limited length, the proceedings of the 16th ESACT meeting that was held in Lugano, Switzerland, in April 1999. We hope it will become a useful resource of the most up-to-date information in animal cell technology, at least until the next meeting in 2001. Classical approaches for the use of animal cells (for example, production of virus vaccines) remain an important technology. However, it appears that major technological advances and major growth are occurring in other areas. Most importantly, protein production on the basis of recombinant DNA molecules transferred into animal cells appears to be an ever-increasing field of interest and innovation. Increasingly animal cells are being used as substrates for the study of gene activation and repression, and also for the more rapid production of small and moderate quantities of interesting proteins. Tissue engineering, somatic gene/cell therapy, organ-replacement technologies, and cell-based bio-sensors all contribute to a considerable widening of interest and research activity, based on animal cell technology.

Table of Contents

16th ESACT Meeting Organising Committee xxi
ESACT Executive Committee xxii
Sponsors xxiii
Companies Participating in the Trade Exhibition xiv
List of Participants
xxv
Introduction Products from Cells-Cellas as Products lxxi
Acknowledgements lxxiii
Improvement and Induction of High Productivity
Recombinant Pharmaceutical Protein Overexpression in an IRF-I Proliferation Controlled Production System
3(8)
Geserick, C.
Schroeder, K.
Bonarius, H.
Kongerslev, L.
Schlenke, P.
Hauser, H.
Mueller, P.P.
The Real Meaning of High Expression
11(8)
Grammatikos, S.I.
Bergemann, K.
Werz, W.
Brax, I.
Bux, R.
Eberhardt, P.
Fieder, J.
Noe, W.
A Novel Mammalian Gene Regulation System Responsive to Streptogramin Antibiotics
19(4)
Fussenegger, M.
Von Stockar, B.
Fux, C.
Rimann, R.
Morris, R.
Thompson, C.J.
Bailey, J.E.
Feedback Control of Redox Potential in Hybridoma Cell Culture
23(8)
Meneses, A.
Gomez, A.
Ramirez, O.T.
Genetic manipulation of the Protein Synthetic Capacity of Mammalian Cells
31(4)
Heaton, M.
Birch, J
Hovey, A.
Kallmeier, R.
Proud, C.
James, D.
Retinoic Acid Enhances Monoclonal Antibody Production of Human-Human Hybridoma BD9
35(4)
Inoue, Y.
Fujisawa, M.
Shoji, M.
Hashizume, S.
Katakura, Y.
Shirahata
Extracellular Adenosine 5' Monophosphate Controls Proliferation and Stimulates Protein Production of rCHO Cells
39(4)
Bonarius, H.P.J.; Hansen, K.; Kongerslev, L.
The Mode of Actions of Lysozyme as an Immunoglobulin Production Stimulating Factor (IPSF)
43(4)
Sugahara, T.
Murakami, F.
Sasaki, T.
A Dissection of Recombinant Protein Expression in CHO Cells Indicating Protein-Specific, not Cell Specific, Limitation
47(4)
Altobello, K.
Chung, YH.
Iovino, J.
Luchette, C.
Overton, S.
Cunningham, M.
Effect of Bioreactor Process Control Parameters on Apoptosis and Monoclonal Antibody Production During the Protein-Free Fed-Batch Culture of a Murine Myeloma
51(4)
Moran, E.
McGowan, St.
Reynolds, N.
Wilson, C.
Adenovirus Vector Production in 293 Fed-Batch Cultures
55(4)
Wong, K.
Guardia, M.J.
Lee, St.
Hu, W.-S.
Influence of BCL-2 Over-Expression on NSO and CHO Culture Viability and Chimeric Antibody Productivity
59(4)
Tey, B.T.
Singh, R.P.
Al-Rubeai, M.
A Regulatable Selective System Facilitating Isolation of High Expression Mammalian Cells
63(4)
Teruya, K.
Zhang, Y.-P.
Katakura, Y.
Shirahata, S.
Comparative Analysis of IRES Efficiency of Dicistronic Expression Vectorsi n Primary Cells and Permanent Cell Lines
67(4)
Schumacher, L.
Alves, P.M.
Wirth, M.
Use of Gel Microdrops Selection System for Recombinant Cells
71(4)
Hansen, K.
Bertelsen, C.
Kongerslev, L.
Bovine Serum-Albumin with Grafted RGD Tailed Cyclic Peptides as an Engineered Proadhesive Protein
75(4)
Dewelle, J.
Delforge, D.
Pirotton, S.
Remacle, J.
Raes, M.
Matrix Attachment Regions in Stable CHO Cell Line Development
79(4)
Zahn, M.M.
Kobr, M.
Mermod, N.
High Cell Density Cultivation of Hybridoma Cells Spin Filter vs Immobilized Culture
83(4)
Heine, H.
Biselli, M.
Wandrey, C.
Influence of the Inoculum on the Production of Antibodies with Mammalian Cells
87(4)
Inglin, M.
Graf-Hausner, U.
Sonnleitner, B.
Spinnler, R.
Leist, Ch.
Establishing and Developing CHO Cell Lines for the Commercial Production of Human Anti-Rhesus D IgG
91(4)
De Jesus, M.
Bourgeois, M.
Jordan, M.
Zahn, M.
Mermod, N.
Amstutz, H.P.
Wurm, F.M.
Glucose and Glutamine Replacement for the Enhancement of Fed-batch Proceses Using CHO Cells
95(4)
Altamirano, C.
Paredes, C.
Cairo, J.J.
Godia, F.
Pilot Scale Production of Human Prothrombin Using a Recombinant CHO Cell Line
99(4)
Lutkemeyer, D.
Heidemann, R.
Tebbe, H.
Gudermann, F.
Lehmann, J.
Schmidt, M.
Raditsch, M.
Maintenance of High Viability During Direct Transition of CHO-KI Cell Growth from 10% to 0% Serum; No Adaptation Required
103(4)
Tsao, M.C.
Johnson, J.M.
Bachelder, C.
Wood, M.A.
Boline, J.A.
Berzofsky, R.N.
Analysis and Simultaneous Isolation of Recombinant t-PA Using High Performance Membrane Affinity Chromatography
107(6)
Renemann, G.
Kretzmer, G.
Tennikova, T.B.
Metabolic and Process Engineering
Improvement of the Primary Metabolism of Cell Cultures Introducing a Pyruvate Carboxylase Reaction Pathway
113(6)
Irani, N.
Wirth, M.
V.d. Heuvel, J.
Beccaria, A.J.
Wagner, R.
Growth Kinetic Studies (In Hours) Based on the Fluorescence of Stable, GFP-Expressing CHO Cells
119(4)
Hunt, L.
Jordan H.
De Jesus, M.
Wurth, F.
Intracellular Localization of Non-Secreted Recombinant ™-Trace Protein in Vesicle-Like Structures of Baculovirus-Infected Insect Cells
123(4)
Schulze, N.
Albers, U.
Grabenhorts, E.
Conradt, H.S.
Rohde, M.
Nimtz, M.
Jager
Induction Kinetics of Apoptosis in Mammalian Cell Cultures
127(4)
Linz, M.
Zeng, A.-P.
Deckwer, W.-D.
A Model for the On-Line Scrutiny of Metabolism: Its Application to the Changing Nutritional Demands of Cultured Animal Cells
131(4)
Guan, Y.H.
Kemp, R.B.
Caspases as a Target for Apoptosis Inhibition in Hybridoma Cells in Culture
135(4)
Gabernet, C.
Tinto, A.
Vives, J.
Prats, E.
Cornudella, L.
Cairo, J.J.
Godia, F.
Cell Activity after Cell Growth Inhibition by the IRF-1 System
139(4)
Coroadinha, A.S.
Carvalhal, A.V.
Moreira, J.L.
Carrondo, M.J.T.
Establishment of a Primary Liver Cell Culture from a Teleost, Oreochromis Mossambicus, the Tilapia: a Valid Tool for Physiological Studies
143(4)
Schmid, A.C.
Kloas, W.
Reinecke, M.
TGB-β Induces Premature and Replicative Senescence in Cancer Cells
147(4)
Miura, T.
Katakura, Y.
Nakata, E.
Uehara, N.
Shirahata, S.
Development of a Fed-batch Strategy that Utilizes Multiple Carbon Sources to Maximize Recombinant Protein Expression in CHO Cells
151(4)
Deer, F.
Cunningham, M.R.
Development of a New, Highly Flexible, Fully Computer Integrated Fermentor Control System
155(4)
Buntemeyer.
Nerkamp, M.
Bockholt, S.
Weber, C.
Gudermann, F.
Lehmann, J.
Cell Growth Rate Estimation in Packed-Bed and Hollow Fibers Bioreactors: Metabolism and Productivity
159(4)
Rodrigues, M.T.A.
Garbuio, A.
Nagao, L.T.
Raw, I.
Moro, A.M.
Influence of Oxygen Limitation on Myeloma Cell Cultures: Various Methods of Inoculum Proliferation for Bioreactor Cultivations
163(4)
Spinnler, R.
Graf-Hausner, U.
Inglin, M.
Leist, Ch.
Comparison of Fed-batch Strategies in Hybridoma Cultures
167(4)
Schwabe, J.O.
Wilkens, I.
Poortner, R.
Non-Invasive Adaptive Control of the Feed Rate for High Cell Density CHO Cultures According to the On-line pO2 Profile
171(4)
Muller, D.
Doblhoff-Dier, O.
Katinger, H.
Cell Growth Control by the IRF-1 System in Perfusion Culture
175(4)
Carvalhal, A.V.
Moreira, J.L.
Carrondo, M.J.T.
Modulation of Cell Cycle for Optimal Recombinant Protein Production
179(4)
Hendrick, V.
Vandeputte, O.
Raschella, A.
Marique, T.
Cherlet, M.
Abdelkafi, C.
Werenne, J.
Application of Bioassays for Suspended 293 Cell Enumerations in Chemically Defined Medium (CDM)
183(4)
Tsao, M.C.
Jacko, B.A.
Curnow, E.
Berzofsky, R.N.
Efficient Cell Growth by an Optimised Protein Free Medium Design and a Controlled Feeding Strategy
187(4)
Loa, A.
Lefebvre, P.
Kretzmer, G.
Development of a Serum Free Medium for a Human Diploid Fibroblast Cell Line
191(4)
Pirotton, S.
Dewelle, J.
Eliaers, F.
Delforge, D.
Raes, M.
The Use of Peptones as Medium Additives for High-Density Perfusion Cultures of Animal Cells
195(4)
Heidemann, R.
Zhang, C.
Qi, H.
Rule, J.L.
Rozales, C.
Park, S.
Chuppa, S.
Ray, M.
Michaels, J.
Konstantinov, K.
Naveh, D.
Determination of Animal Cell Densities and Viability by the New Analyser CEDEX2
199(4)
When, T.
Lorenz, Th.
Behrendt, U.
Wallerius, C.
Buntemeyer, H.
Lehmann, J.
Determination of Animal Cell Densities and Viability; Comparison of the Microcyte Flow Cytometer with Trypan Blue Counts and Coulter Count
203(4)
Vriezen, N.
Mid-Infrared Spectroscopic Monitoring of Animal Cell Culture Bioractor Processes
207(4)
Rhiel, M.
Ziegler, T.
Ducommun, P.
Von Stockar, U.
Marison, I.W.
Mammalian Cell Monitoring using the Microcyte Flow Cytometer
211(4)
Harding, C.L.
Lloyd, D.r.
McFarlane, C.M.
Al-Rubeai, M.
Indirect Biomass Determination in Case of Non-Constant Metabolic Rates
215(4)
Ducommun, P.
Ziegler, T.
Rhiel, M.
Von Stockar, U.
Marison, I.W.
Whole Cell -- Smart Microsensor: A first Approach Towards Interfacing Live Cells and Electronics
219(4)
Charlier, O.
Goffaux, M.-J.
Marique, T.
Hendrick, V.
De Vos, L.
Werenne, J.
Comparison of Immobilised Group Specific Affinity Ligands for the Bioseparation of Antibodies by High Performance Membrane Affinity Chromatography
223(6)
Berruex, L.G.
Freitag, R.
Post-Translational Modifications
Posttranslational Modifications Limit High Level Expression of Functionally Active Chimeric P-Selectin Glycoprotein Ligand-1 in rCHO Cells
229(8)
Sinacore, M.S.
Richards, T.
Francullo, L.
Woodard, A.
Hardy, M.
Cornell, R.
Koza, S.
Davies, M.
Ellis, D.
Harrison, S.
Glycolsylation Analysis of Nanomolar Amounts of Glycoprotein Comtining In-gel Enzymatic Digestion and FACETM
237(4)
Kloth, C.
Leibiger, H.
Valley, U.
Yalcin, E.
Buchholz, R.
Emmrich, F.
Marx, U.
Glycosylation Patterns of a rec-Fusion Protein Expressed in BHK Cells at Different Metabolic States
241(4)
Cruz, H.J.
Conradt, H.S.
Peixoto, C.M.
Alves, P.M.
Nimtz, M.
Dias, E.M.
Santos, H.
Moreira, J.L.
Carrondo, M.J.T.
Elimination of N-Glycolylneuraminic Acid Residues in Recombinant Glycoproteins: A Gene Knock-Out Approach
245(6)
Fontana, O.
Covini, N.
Pighini, M.
Carpani, D.
Buono, P.
Soria, M.R.
Monaco, L.
Fucosyltransferase III Production by Mammalian and Insect Cells for the Synthesis of Therapeutic Oligosaccharides
251(4)
Benslimane, C.
Chenu, S.
Tahrat, H.
Deparis, V.
Auge, C.
Cerutti, M.
Delannoy, P.
Goergen, J.L.
Marc, A.
Control of Therapeutic Monoclonal Antibody Glycosylation
255(4)
Hills, A.E.
Patel, A.K.
Boyd, P.N.
James, D.C.
The Effect of Cell Line, Transfection Procedure and Reactor Conditions on the Glycosylation of Recombinant Human Anti-Rhesus D IgGI
259(4)
Nahrgang, S.
Kragten, E.
De Jesus, M.
Bourgeois, M.
Dejardin, S.
Von Stockar, U.
Marison, I.W.
Metabolic Engineering of Recombinant Plasminogen Sialylation in Animal Cells by Tetracycline-Regulated Expression of Murine CMP-NeuAc Synthetase and Hamster CMP-NeuAc Transporter Genes
263(6)
Baker, K.N.
Johnson, I.D.
Roberts, G.
Cook, A.
Baines, A.
Gerardy-Schahn, R.
James, D.C.
Reactor Design and Operation
Air-Trap-Technology: Bubble-Free Gas Transfer in Large Scale
269(8)
Dascher, M.B.
Sonnleitner, B.
Georg, A.
The Improvement of Aeration in 8000 I Animal Cell Culture Vessels
277(8)
Clayton, T.M.
Jenkins, I.
Steward, P.
A Novel Continuous Suspension Culture System for Hematopoietic Cells
285(4)
Schmidt, S.
Jelinek, N.
Hilbert, U.
Thoma, S.
Wandrey, C.
Biselli, M.
Large Scale Production of HIV-I gpl20 Using Recombinant Vaccinia Virus in Packed-Bed Bioreactor
289(4)
Kaufman, J.
Cho, M.W.
Lee, M.K.
Shiloach, J.
The ``Shear Tester''. A Universal Tool for Characterization of the Shear Sensitivity of Animal Cell Lines under Various Process Conditions
293(6)
Flagmeyer, A.
Konstantinov, K.
Henzler, H.J.
Membrane Oxygenation of Mammalian Cell Culture Fermenters Using DuPont Teflon AF-2400 Tubing
299(4)
Blackie, J.
Wu, P.
Naveh, D.
Configuration of a Simple Dialysis Membrane Bioreactor Suitable for Large-Scale Application
303(4)
Kleuser, B.
Kocher, H.P.
Memmert, K.
Stenz, M.
Straubhaar, B.
Zurini, M.
Erythropoietin Production from CHO Cells Grown in a Fluidized-Bed Bioreactor with Macroporous Beads
307(4)
Wang, M.D.
Yang, M.
Huzel, N.
Butler, M.
Scale-Up of Fixed-Bed Reactors for the Cultivation of Animal Cells
311(4)
Fassnacht, D.
Reimann, I.
Portner, R.
A Novel Conical Shaped Bioreactor for Small-Scale Investigations under Defined Conditions
315(4)
Schwabe, J.O.
Matsuoka, H.
Jungkeit, H.
Portner, R.
Continuous Perfused Fluidized Bed Technology: Increased Productivities and Product Concentrations
319(4)
Muller, D.
Unterluggauer, F.
Kreismayr, G.
Schmatz, C.
Wiederkum, S.
Preis, S.
Vorauer-Uhl, K.
Assadian, A.
Doblhoff-Dier, O.
Katinger, H.
Small Scale Bioreactor System for Process Development and Optimization
323(8)
Girard, P.
Meissner, P.
Jordan, M.
Tsao, M.
Wurm, F.
Transient Gene Expression
Mammalian Transient Gene Expression-Potential, Practical Applications and Perspectives
331(8)
Blasey, H.D.
Hovius, R.
Rey, L.
Vogel, H.
Bernard, A.R.
Float!--And Express More: Converting the HEK.EBNA/oriP System to Suspension Culture
339(8)
Geisse, S.
Orakci, O.
Pabler, A.
Patoux, A.
Rinaldi, D.
Watkins, J.
Schmitz, R.
Gram, H.
Multiplicity and Time of Infection as Tools to Maximize Virus-Like Particle Production by Insect Cells
347(4)
Palomares, L.A.
Lopez, S.
Arias, C.F.
Ramirez, O.T.
Process Development for Transient Gene Expression in Mammalian Cells at the 3 L Scale: 10-50 MG of R-Protein in Days
351(8)
Meissner, P.
Girard, P.
Kulangara, A.
Tsao, M.C.
Jordan, M.
Wurm, F.M.
A Method to Determine the Optimal Time to Infect Insect Cells with the Baculovirus Expression System
359(4)
Ljunggren, J.
Alarcon, M.
Ramqvist, A.K.
Westlund, A.
Ohman, L.
Analysis of Baculovirus Infected Sf9 Cells Using Flow Cytometry
363(4)
Pettman, G.R.
Adaptation of Recombinant HEK-293 Cells to Growth in Serum-Free Suspension
367(4)
McAllister, R.C.
Schofield, C.
Pettman, G.R.
Mannix, C.
Growth on Microcarriers and Nutritional Needs of High Density Insect Cell Cultures
371(4)
Ikonomou, L.
Bastin, G.
Schneider, Y. J.
Agathos, S.N.
Use of Flow Cytometry in the Evaluation of Hydroxyapatite for the Purification of DNA Plasmid
375(6)
Wright, J.L.
Egtard, P.
Van Adrichen, J.
Freitag, R.
Wurm, F.M.
Regulatory and Stability Issues in Animal Cell Culture for Pharmaceutical Production
Regulation and Standardisation of Gene Transfer Products in the European Union
381(8)
K. Cichutek
Study of Stability of Expression of a Humanized Monoclonal Antibody from a Transfected NSO Myeloma Cell Line Using Different Culture Media and Serum Concentrations
389(4)
Castillo, A.J.
Victores, S.
Marison, I.W.
Analysis of Alterations in Gene Expression after Amplification of Recombinant Genes in CHO Cells
393(4)
Hohenwarter, O.
Grillari, J.
Fortschegger, K.
Grabherr, R.M.
Katinger, H.
Stable Recombinant Expression and Functional Identiy of the Anti HIV-I Monoclonal Antibody 2F5 after IGG3/IGGI Subclass Switch in CHO-Cells
397(4)
Kunert, R.
Steinfellner, W.
Assadian, A.
Katinger, H.
Analysis of Genomic DNA and RNA-Transcripts of a Heterohybridoma Simultaneously Expressing IgG3 and IgGI with Identical Specificity
401(6)
Kunert, R.
Wolbank, S.
Huser, A.
Katinger, H.
Novel Therapeutic and Prophylactic Approaches Based on Cells and Nucleic Acids
Tissue Engineering of a Bladder Wall Patch
407(6)
Bisson, I.
Hilborn, J.
Wurm, F.
Frey, P.
Integrated Optimization of Retrovirus Production for Gene Therapy
413(8)
Cruz, P.E.
Goncalves, D.
Almeida, J.
Moreira, J.L.
Carrondo, M.J.T.
Toxicological Evaluation of Surface Water Samples in Sensitized Cultured Fish Cells, and Comparison with the Microtox® Method
421(4)
Dierickx, P.J.
Van der Wielen, C.
Francois, N.
Electrolyzed and Natural Reduced Water Exhibit Insulin-Like Activity on Glucose Uptake into Muscle Cells and Adipocytes
425(4)
Oda, M.
Kusumoto, K.
Teruya, K.
Hara, T.
Maki, T.
Kabayama, S.
Katakura, Y.
Otsubo, K.
Morisawa, S.
Hayashi, H.
Ishii, Y.
Shirahata, S.
Study of DDS for a Cancer Therapy Applying Lipid Vesicle Immobilizing Eucheuma Serra Lectin
429(4)
Kato, K.
Sugahara, T.
Maruyama, Y.
Yoshimura, N.
Yoshihiro, A.
Tateishi, N.
Suzuki, Y.
Kawakubo, A.
Sasaki, T.
Study of the Specific Binding Between Lipid Vesicles and Human-Human Hybridoma toward Either DDS or Gene Transfection
433(4)
Kato, K.
Sugahara, T.
Kawashima, S.
Hayashi, Y.
Yoshihiro, A.
Sasaki, T.
Suppression of Apoptosis in UV-Damaged Human Melanoma Cells by a Fermented Milk, Kefir
437(4)
Nagira, T.
Narisawa, J.
Teruya, K.
Kusumoto, K.
Katakura, Y.
Barnes, D.W.
Tokumaru, S.
Shirahata, S.
Large Scale GMP Production of Retroviral Gene Therapy Vector in Very Low Serum Medium
441(6)
Moy, N.
Dillingham, M.
McGowan, S.
Rotherham, M.
Clayton, T.M.
Vaccines and Immunologicals
Chimeric Lyssavirus Glycoprotein: New Vector for Multivalent Vaccines
447(8)
Desmezieres, E.
Jacob, Y.
Saron, M. F.
Delpeyroux, F.
Tordo, N.
Perrin, P.
The SPP Biotech Module 1: Proteins for Medical Applications. A Multidisciplinary Effort for the Development of a Recombinant Anti-RhD Antibody Preparation of Hemolytic Disease of the Newborn
455(4)
Amstutz, H.
Miescher, S.
Moudry, R.
Dejardin, S.
Imboden, M.
Morgenthaler, J.J.
Zahn, M.
Kobr, M.
Fisch, I.
Corthesy, B.
Mermod, N.
Berruex, L.
Abdellali, M.
Bruggemann, O.
Fraboulet, D.
Freitag, R.
Kragten, E.
Nahrgang, S.
Ducommun, P.
Pugeaud, P.
Marison, I.
Von Stockar, U.
De Jesus, M.
Jordan, M.
Bourgeois, M.
Hunt, L.
Radice, S.
Baldi, L.
Kulangara, A.
Girard, P.
Ribicki, R.
Wurm, F.
Low Serum and Serum-Free Cultivation of Mammalian Cells Used for Virus Production Applications
459(4)
Jayme, D.W.
Price, P.J.
Plavsic, M.Z.
Epstein, D.A.
Development of a Serum Free Medium for MRC-5 Culture
463(4)
Maggetto, C.
Pirotton, S.
Knott, I.
Aerts, B.
Evrard, E.
Gonze, M.M.
Raes, M.
Fabry, L.
Adaptation of Vero Cells to a Serum Free Medium for the Production of Rabies Virus
467(4)
Majoul, S.
Kharmachi, H.
Saadi, M.
Chouaib, A.
Kallel, H.
Increased Productivity of a Rickettsia Vaccine: Enhancement of Adhesion Properties of Infected Endothelial Cells by TGFB
471(4)
Marique, T.
Ndongo, D.E.
Tanga, M.
Raschella, A.
Henrick, V.
Declerq, B.
Werenne, J.
Comparative Study of Cell Cultures Perspective for Rubella Virus Production
475(4)
Getmanova, T.N.
Chepurnov, A.A.
Makhova, N.M.
Nechaeva, E.A.
Kolokoltsova, T.D.
Biogenesis of Epithelial Phenotype During Long-Term Cultivation of MDCK Cells in Serum-Free Ultra-MDCK Medium
479(4)
Kessler, N.
Thomas, G.
Aymard, M.
Sandwich Elisa Today; Limits for Sensitivity, Speed, Precision and Throughput
483(6)
Jordan, M.
Fraboulet, D.
Fourmestraux, G.
Wurm, F.
Freitag, R.
Cell Characterization, Validation, Safety, Adventitious Agents
Impact of ICH Guidelines on Phases of Development of Biotechnology Products
489(6)
Lubiniecki, A.S.
The Microbial Risks of Xenotransplantation
495(6)
Galbraith, D.N.
Onions, D.E.
Cryopreservation under Protein-Free Medium Conditions: A Reliable Way to Safe Cell Banking
501(4)
Hesse, F.
Scharfenberg, K.
Wagner
Creation of Certified Cell Banks Suitable for Production of Healing and Immunobiological Preparations
505(4)
Kolokoltosva, T.D.
Yurtchenko, N.D.
Isaenko, A.A.
Nechaeva, E.A.
Shurnakova, O.V.
Getmanova, T.N.
Endogeneous Retrovirus Particles and Their Effects on the Growth Behavior of Hybridoma Cultivation Processes
509(4)
Hawerkamp, A.
Lutkemeyer, D.
Falkenhain, A.
Gudermann, F.
Buntemeyer, H.
Lehmann, J.
Testing for Adventitious Retroviruses Using PCR-Based Reverse Transcriptase Assays
513(4)
Lovatt, A.
Black, J.
Galbraith, D.
Doherty, I.
Moran, M.W.
Wilson, N.
Shepherd, A.J.
Smith, K.T.
Rapid RNA Quantitation from Xenotropic SP2/0 Retroviruses by Competitive RT-PCR ELISA
517(4)
Hansen, W.
Schumacher, L.
Maeggi, M.C.
Knopf, H.P.
Wirth, M.
Human Amniotic Epithelial (HAE) Cells Express Erythropoietin (EPO) and EPO-Receptor
521(4)
Katsuura, K.
Hoshika, A.
Terada, S.
Sakuragawa, N.
The Nordenau-Phenomenon -- Facts and Hypotheses
525(4)
Gadek, Z.
Zeifert, J.D.
Conversion of a Process Development Pilot Plant into a Clinical Phase I/II Manufacturing Facility for Recombinant Proteins Expressed in Mammalian Cells
529(6)
Klamer, T.
Petersen, J.M.
Skovgaard, R.
Animal or Patient Derived Cells and Tissues for Therapeutic Applications
Human Keratinocyte Culture on Macroporous Carriers at the Air-Liquid Interface
535(4)
Linnau, E.K
Burt, A.M.
Katinger, H.
Handa-Corrigan, A.
Comparison of Viability and Function of Primary Cells with Transformed Cells for Use in a Bioartificial Liver Support System
539(4)
Wang, L.
Li, L.
Sun, J.
Mears, D.
Horvat, M.
Sheil, A.G.R.
Harbour, C.
Determining Optimal Transport Conditions of In Vitro Cultured Human Chondrocytes Prepared for Autologous Re-Implantation Using Flow Cytometry
543(4)
Wozniak, G.
Velikonja, N.
Knezevic, M.
Urbajs, M.
Jeras, M.
Radosavlejevic, D.
Rozman, P.
Effects of Collagenase on Human Hematopoietic Cells
547(4)
Jelinek, N.
Schmidt, S.
Thoma, S.
Wandrey, C.
Biselli, M.
Validation of the CACO-2 Test for Intestinal Permeability Assessment of Drugs: the Usefulness of PAPP, the Apparent Permeability Coefficient
551(4)
Fassio, F.
Nolli, M.L.
Golzio, L.
Bertolino, M.
Canali, S
Barone, D.
Author Index 555(6)
Subject Index 561

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