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9780121820657

Ribosomes

by ; ; ;
  • ISBN13:

    9780121820657

  • ISBN10:

    0121820653

  • Format: Hardcover
  • Copyright: 1988-12-28
  • Publisher: Elsevier Science

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Supplemental Materials

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Summary

This volume includes a variety of methods involving electron microscopy and other biophysical methods, such as crystallography, neutron scattering, and NMR procedures for the analysis of protein-RNA or RNA-RNA interactions by cross-linking, the use of chemical, enzymatic, and immunological probes, as well as functional and genetic approaches for the study of this nucleoprotein. These methodologies will contribute to the progress toward the elucidation of the structure, function, and regulatory processes that affect this most important complex cellular component, the ribosome.

Table of Contents

Electron Microscopy
Studying Ribosome Structure by Electron Microscopy and Computer-Image Processing
Statistical Image Analysis of Electron Micrographs of Ribosomal Subunits
Structural Analysis of Ribosomes by Scanning Transmission Electron Microscopy
Identification of Protein-Protein Cross-Links within the Escherichia coli Ribosome by Immunoblotting Techniques
Electron Microscopy Studies of Ribosomal RNA. Other Biophysical Methods
Crystallographic and Image Reconstruction Studies on Ribosomal Particles from Bacterial Sources
Neutron-Scattering Topography of Proteins of the Small Ribosomal Subunit
Preparation and Activity Measurements of Deuterated 50S Subunits for Neutron-Scattering Analysis
Nuclear Magnetic Resonance Techniques for Studying Structure and Function of Ribosomes
Preparation of 5S RNA-Related Materials for Nuclear Magnetic Resonance and Crystallography Studies
Fluorescence Labeling and Isolation of Labeled RNA and Ribosomal Proteins
Isolation and Characterization of Colicin Fragments of Bacterial 16S Ribosomal RNA
Protein-RNA Interactions
Physical Studies of Ribosomal-Protein-RNA Interactions
Preparation of Specific Ribosomal RNA Fragments
Physical Studies of the Interaction of Escherichia coli Translational Initiation Factor 3 Protein with Ribosomal RNA
Isolation of Fragments of Ribosomal Proteins That Recognize rRNA
Isolation of Kinetic Intermediates in in Vitro Assembly of the Escherichia coli Ribosome Using Cibacron Blue F3GA
Total Reconstitution of 70S Ribosomes from Escherichia coli
Cross-Linking and Affinity-Labeling Methods
Intra-RNA and RNA-Protein Cross-Linking Techniques in Escherichia coli Ribosomes
RNA-Protein Cross-Linking
Isolation and Identification of RNA Cross-Links
Psoralen Cross-Linking of Ribosomal RNA
Affinity Labeling of Ribosomes
Photoaffinity Labeling of Peptidyltransferase
Affinity Labeling of tRNA-Binding Sites on Ribosomes
Chemical and Enzymatic Probing Methods
Probing Ribosome Structure and Function Using Short Oligodeoxyribonucleotides
Extension Inhibition Analysis of Translation Initiation Complexes
Table of Contents provided by Publisher. All Rights Reserved.

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