This volume presents the latest science on the regulation of phosphate metabolism in E. coli, Saccharomyces cerevisiae, and other important microbial systems. Based on a meeting held at Woods Hole, Massachusetts (September 1993), this book covers exciting new findings on the role of phosphate in signal transduction, protein structure, genetic control pathogenesis, and protein export and folding. Phosphate in Microorganisms will prove to be significant reading for anyone interested in bacterial metabolism and molecular biology of prokaryotic and lower eukaryotic organisms.

Preface
Acknowledgments
Sketch of Woods Hole
Participants
Introduction: The Pho Regulon of Escherichia coli	p. 1
Mechanism of Transcriptional Activation of the Phosphate Regulon in Escherichia coli	p. 5
Multiple Controls of the Escherichia coli Pho Regulon by the P[subscript i] Sensor PhoR, the Catabolite Regulatory Sensor CreC, and Acetyl Phosphate	p. 13
Phosphate, Phosphorylated Metabolites, and the Pho Regulon of Escherichia coli	p. 22
The Ugp Paradox: the Phenomenon That Glycerol-3-Phosphate, Exclusively Transported by the Escherichia coli Ugp System, Can Serve as a Sole Source of Phosphate but Not as a Sole Source of Carbon Is Due to trans Inhibition of Ugp-Mediated Transport by Phosphate	p. 30
Proposed Mechanism for Phosphate Translocation by the Phosphate-Specific Transport (Pst) System and Role of the Pst System in Phosphate Regulation	p. 37
Phosphate Inorganic Transport (Pit) System in Escherichia coli and Acinetobacter johnsonii	p. 43
The Pho Regulon of Bacillus subtilus Is Regulated by Sequential Action of Two Genetic Switches	p. 50
Introduction: Regulation of Phosphate Metabolism in Saccharomyces cerevisiae	p. 55
Regulatory Circuit for Phosphatase Genes in Saccharomyces cerevisiae: Specific cis-Acting Sites in PHO Promoters for Binding the Positive Regulator Pho4p	p. 56
Protein-DNA and Protein-Protein Interactions Regulating the Phosphatase Multigene Family of Saccharomyces cerevisiae	p. 63
Characterization of Pho85 Kinase of Saccharomyces cerevisiae	p. 70
Introduction: Honorary P[subscript i]	p. 77
Transmembrane Control of the Uhp Sugar-Phosphate Transport System: the Sensation of Glu6P	p. 78
Finding the Hole in UhpT: Applications of Molecular Biology to a Membrane Carrier	p. 85
Maltose Transport System of Escherichia coli as a Member of ABC Transporters	p. 91
The Arsenite Oxyanion-Translocating ATPase: Bioenergetics, Functions, and Regulation	p. 97
Selenophosphate: Synthesis, Properties, and Role as Biological Selenium Donor	p. 109
Specific and General Responses of Cyanobacteria to Macronutrient Deprivation	p. 112
Introduction: Phosphate and Survival of Bacteria	p. 119
The Salmonella PhoP Virulence Regulon	p. 120
Phosphate and Osmoprotectants in the Pathogenesis of Pseudomonas aeruginosa	p. 126
Integration of Multiple Developmental Signals by the Phospho-Transfer Pathway That Controls the Initiation of Sporulation in Bacillus subtilus	p. 133
Phosphate Control of Antibiotic Biosynthesis at the Transcriptional Level	p. 140
Introduction: Bacterial Phosphotransferase Systems	p. 149
The Bacterial Phosphoenolpyruvate: Glycose Phosphotransferase System	p. 151
Model for the Role of Domain Phosphorylation in the Mechanism of Carbohydrate Transport via Enzyme II of the Phosphoenolpyruvate-Dependent Mannitol Phosphotransferase System	p. 161
Enzymes II of the Phosphotransferase System: Transport and Regulation	p. 169
Modular Structure of the Enzymes II of Bacterial Phosphotransferase Systems	p. 175
Signal Transduction through Phosphotransferase Systems	p. 182
The Bacterial Phosphotransferase System: a Multifaceted Regulatory System Controlling Carbon and Energy Metabolism	p. 189
Introduction: Polyphosphates and Phosphate Reserves	p. 195
Involvement of the Quinoprotein Glucose Dehydrogenase in the Solubilization of Exogenous Mineral Phosphates by Gram-Negative Bacteria	p. 197
Inorganic Polyphosphate: a Molecular Fossil Come to Life	p. 204
Molecular Genetics of Polyphosphate Accumulation in Escherichia coli	p. 209
Phosphate-Regulated Genes for the Utilization of Phosphonates in Members of the Family Enterobacteriaceae	p. 215
Introduction: Lipid Involvement in Protein Translocation in the Prokaryotic Secretion Pathway	p. 223
Roles of Phospholipids in Escherichia coli	p. 230
Role of Negatively Charged Phospholipids in the Mode of Action of Pore-Forming Colicins: an Attempt to Relate In Vitro and In Vivo Studies	p. 239
Introduction: Protein Export and Folding	p. 245
Membrane Protein Assembly: Can Protein-Lipid Interactions Explain the "Positive Inside" Rule?	p. 247
Recognition of Ligands as Nonnative by SecB, a Molecular Chaperone Involved in Export of Protein in Escherichia coli	p. 251
Molecular Mechanism of Protein Translocation across the Cytoplasmic Membrane of Escherichia coli	p. 258
Escherichia coli Alkaline Phosphatase Biogenesis: Influence of Overproduction and Amino Acid Substitutions	p. 264
Pathways of Disulfide Bond Formation in Proteins In Vivo	p. 270
Biogenesis of Outer Membrane Porin PhoE of Escherichia coli	p. 276
Introduction: Signal Transduction and Phosphoproteins	p. 282
Signal Transduction in the Phosphate Regulon of Escherichia coli: Dual Functions of PhoR as a Protein Kinase and a Protein Phosphatase	p. 285
Role of Protein Phosphorylation in the Regulation of Aerobic Metabolism by the Arc System in Escherichia coli	p. 290
Role of Histidine Protein Kinases and Response Regulators in Cell Division and Polar Morphogenesis in Caulobacter crescentus	p. 296
Regulation of Bacterial Nitrogen Assimilation by the Two-Component System NR[subscript I] (NtrC) and NR[subscript II] (NtrB)	p. 302
Structural Basis for the Mechanism of Phosphoryl Transfer in Bacterial Chemotaxis	p. 309
Phosphate Taxis and Its Regulation in Pseudomonas aeruginosa	p. 315
Introduction: Structure and Function of Escherichia coli Alkaline Phosphatase	p. 319
Phosphoporin: a Catalog of Open Questions	p. 329
Zn(II)-Mediated Protein Interactions in Escherichia coli Signal Transduction: Cation-Promoted Association of the Phosphotransferase System Regulatory Protein III[superscript Glc] with Target Protein Glycerol Kinase	p. 335
Author index	p. 343
Subject index	p. 345
Table of Contents provided by Blackwell. All Rights Reserved.

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Phosphate in Microorganisms

9781555810801

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