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Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.
Stefan Przyborski is Professor of Cell Technology in the Department of Biosciences, Durham University, UK. He is also the Chief Scientific Officer for ReproCELL Europe, UK. A recognised leader in the field of 3D cell culture, Professor Przyborski has published extensively in this field and presents his work regularly at international events.
List of contributors, vii
Preface, xi
List of abbreviations, xiv
1 An introduction to the third dimension for routine cell culture, 1Antonio Romo‐Morales and Stefan Przyborski
Part I: Aggregate‐based technologies
2 Gravity‐enforced microtissue engineering, 23Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm
3 Physiologically relevant spheroid models for three‐dimensionalcell culture, 50Nicole A. Slawny and MaryAnn Labant
4 NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture, 74Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman
5 Micro‐moulded non‐adhesive hydrogels to form multicellular microtissues – the 3D Petri Dish®, 97Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan
6 Organotypic microtissues on an air‐liquid interface, 123Lars E. Sundstrom, Igor Charvet and Luc Stoppini
Part II: Hydrogels
7 Materials and assay systems used for three‐dimensional cell culture, 145Suparna Sanyal and Marshall Kosovsky
8 HyStem®, a customisable hyaluronan‐based hydrogel matrix for 3D cell culture, 173T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach‐Carson and D. D. Carson
9 3‐D Life biomimetic hydrogels: A modular system for cell environment design, 197Brigitte M. Angres and Helmut Wurst
Part III: Scaffolds
10 Alvetex®, a highly porous polystyrene scaffold for routine three‐dimensional cell culture, 225Antonio Romo‐Morales, Eleanor Knight and Stefan Przyborski
11 CelluSponge™ and Go Matrix as innovative three‐dimensional cell culture platforms, 250Bramasta Nugraha
12 Mimetix® electrospun scaffold: An easy‐to‐use tool for 3D cell culture in drug discovery and regenerative medicine, 284Robert J. McKean and Elena Heister
Part IV: 3D bioreactor technologies
13 Quasi Vivo® bioreactor technology, 305J. Malcolm Wilkinson
14 Three‐dimensional cell‐based assays in hollow fibre bioreactors, 327John J. S. Cadwell and William G. Whitford
15 Three‐dimensional engineered tissues for high‐throughput compound screening: Mechanical properties of skin and ageing, 351Michael Conway, Ayla Annac and Tetsuro Wakatsuki
16 Three‐dimensional cell culture in the Rotary Cell Culture System™, 370Stephen S. Navran
Glossary, 386
Index, 393
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